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dab kit (mouse monoclonal)  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology dab kit (mouse monoclonal)
    Dab Kit (Mouse Monoclonal), supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dab+kit+(mouse+monoclonal)/dab+kit/pm23270819-44-15-19
    Average 90 stars, based on 1 article reviews
    dab kit (mouse monoclonal) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Immunohistochemical staining:

    Article Title: GPC-1 may serve as a predictor of perineural invasion and a prognosticator of survival in pancreatic cancer.
    Article Snippet: For immunohistochemical analysis, the sections were stained by streptavidin-peroxidase method with the use of a DAB kit (mouse monoclonal, Santa Cruz Biotechnology, United States of America).

    Staining:

    Article Title: GPC-1 may serve as a predictor of perineural invasion and a prognosticator of survival in pancreatic cancer.
    Article Snippet: For immunohistochemical analysis, the sections were stained by streptavidin-peroxidase method with the use of a DAB kit (mouse monoclonal, Santa Cruz Biotechnology, United States of America).



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    Image Search Results


    Figure 5. miR-125b promotes apoptosis by targeting Mcl-1, Bcl-w and IL-6R. (a) miR-125b suppressed constitutive and IL-6-induced phosphorylation of Stat3. Forty-eight hours after transfection, SMMC7721 cells were incubated in serum-free Dulbecco’s modified Eagle’s medium (DMEM) with or without 50 ng/ml IL-6 for 30 min, followed by western blotting. b-Actin, internal control. (b) miR-125b suppressed IL-6-induced phosphorylation and nuclear translocation of Stat3. SMMC7721 cells transfected with the indicated RNA were treated with 50 ng/ml IL-6 for 30 min and then analyzed by immunofluorescent staining. The nuclei were stained blue with 40-60-diamidino-2-phenylindole (DAPI). Scale bar, 20 mm. (c) Restoration of miR-125b or silencing of IL-6R diminished the expression of Mcl-1 and Bcl-xL in IL-6-exposed cells. Twenty-four hours after transfection, SMMC7721 cells were cultured in serum-free DMEM for 20 h and then exposed to 10 ng/ml IL-6 for 4 h before western blotting. (d) miR-125b overexpression reduced the levels of Mcl-1, Bcl-xL and Bcl-w. SMMC7721 cells were transfected with miR-125b or siIL-6R for 48 h before western blotting. b-Actin, internal control. (e) Knockdown of Mcl-1, Bcl-w and IL-6R-promoted apoptosis. Twenty-four hours after transfection with the indicated single siRNA (50 nM) or siRNA mixture (16.7 nM of each siMcl-1, siBcl-w and siIL-6R), SMMC7721 cells were deprived of serum for 72 h and then subjected to DAPI staining. **Po0.01; ***Po0.001.

    Journal: Oncogene

    Article Title: MicroRNA-125b promotes apoptosis by regulating the expression of Mcl-1, Bcl-w and IL-6R.

    doi: 10.1038/onc.2012.318

    Figure Lengend Snippet: Figure 5. miR-125b promotes apoptosis by targeting Mcl-1, Bcl-w and IL-6R. (a) miR-125b suppressed constitutive and IL-6-induced phosphorylation of Stat3. Forty-eight hours after transfection, SMMC7721 cells were incubated in serum-free Dulbecco’s modified Eagle’s medium (DMEM) with or without 50 ng/ml IL-6 for 30 min, followed by western blotting. b-Actin, internal control. (b) miR-125b suppressed IL-6-induced phosphorylation and nuclear translocation of Stat3. SMMC7721 cells transfected with the indicated RNA were treated with 50 ng/ml IL-6 for 30 min and then analyzed by immunofluorescent staining. The nuclei were stained blue with 40-60-diamidino-2-phenylindole (DAPI). Scale bar, 20 mm. (c) Restoration of miR-125b or silencing of IL-6R diminished the expression of Mcl-1 and Bcl-xL in IL-6-exposed cells. Twenty-four hours after transfection, SMMC7721 cells were cultured in serum-free DMEM for 20 h and then exposed to 10 ng/ml IL-6 for 4 h before western blotting. (d) miR-125b overexpression reduced the levels of Mcl-1, Bcl-xL and Bcl-w. SMMC7721 cells were transfected with miR-125b or siIL-6R for 48 h before western blotting. b-Actin, internal control. (e) Knockdown of Mcl-1, Bcl-w and IL-6R-promoted apoptosis. Twenty-four hours after transfection with the indicated single siRNA (50 nM) or siRNA mixture (16.7 nM of each siMcl-1, siBcl-w and siIL-6R), SMMC7721 cells were deprived of serum for 72 h and then subjected to DAPI staining. **Po0.01; ***Po0.001.

    Article Snippet: Reagents The reagents used were as follows: rabbit polyclonal antibodies for Mcl-1 (sc-819, Santa Cruz Biotechnology, Santa Cruz, CA, USA), Bcl-w (sc-130701), IL-6R (sc-661, Santa Cruz Biotechnology), caspase-3 (no. 9662, Cell Signaling Technology, Beverly, MA, USA); mouse monoclonal antibodies against Tyr705-p-Stat3 (sc-8059), Stat3 (sc-8019), Bcl-xL (sc-8392), p53 (sc-126, Santa Cruz Biotechnology) and b-actin (BM0627, Boster, Wuhan, China); ECL kit (Pierce, Rockford, IL, USA); 40-60-diamidino-2-phenylindole (Sigma-Aldrich, St Louis, MO, USA); IL-6 (206-IL, R&D Systems, Oxon, UK); cell culture plates (Corning Glass Works, Corning, NY, USA).

    Techniques: Phospho-proteomics, Transfection, Incubation, Western Blot, Control, Translocation Assay, Staining, Expressing, Cell Culture, Over Expression, Knockdown